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anti fading mounting solution  (Beyotime)


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    Structured Review

    Beyotime anti fading mounting solution
    Anti Fading Mounting Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2060 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+fade+mounting+solution/DAPI+Staining+Solution/pmc12269989-359-17-21
    Average 99 stars, based on 2060 article reviews
    anti fading mounting solution - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Honokiol Eliminates Glioma/Glioblastoma Stem Cell-Like Cells Via JAK-STAT3 Signaling and Inhibits Tumor Progression by Targeting Epidermal Growth Factor Receptor.
    Article Snippet: Cells were fixed and incubated with antibodies against CD133/1 (1:50; Cat. No. 18470-1-AP, Proteintech) and Nestin (1:50; Cat. No. 66259-1-Ig, Proteintech) overnight at 4 ◦C After washing in PBS three times for 5 min each, the samples were incubated at room temperature with goat anti-mouse fluorescein (FITC)–conjugated antibody (1:500; Jackson ImmunoResearch) and anti-rabbit secondary antibody conjugated to DylightTM 594 (1:500; Jackson ImmunoResearch) for 60 min. .. The cells were then stained with DAPI and mounted using anti-fade mounting solution (Beyotime). .. Cell lysates were heated at 100 ◦C in sample loading buffer (0.125 mol/L Tris-HCl, pH 6.8, 20% glycerol, 10% SDS, 0.1% bromophenol blue and 5% β-mercaptoethanol), resolved by 10% SDS polyacrylamide gel electrophoresis and electroblotted onto polyvinylidene difluoride membranes (PVDF, Merck KGaA, Darmstadt, Germany).

    Article Title: Honokiol Eliminates Glioma/Glioblastoma Stem Cell-Like Cells Via JAK-STAT3 Signaling and Inhibits Tumor Progression by Targeting Epidermal Growth Factor Receptor.
    Article Snippet: After washing in PBS three times for 5 min each, the samples were incubated at room temperature with FITC-conjugated goat anti-mouse IgG antibody (1:500; Jackson ImmunoResearch, West Grove, PA, USA) for 60 min at room temperature. .. The cells were then stained with 4′,6-diamidino-2-phenylindole (DAPI) and mounted using anti-fade mounting solution (Beyotime). .. CD133+ cells were seeded onto glass coverslips coated with poly-L-lysine (Sigma-Aldrich).

    Article Title: Akkermansia muciniphila Is Beneficial to a Mouse Model of Parkinson’s Disease, via Alleviated Neuroinflammation and Promoted Neurogenesis, with Involvement of SCFAs
    Article Snippet: The sections were washed three times in PBS and treated for 1 h at 37 °C with a secondary antibody coupled to FITC-conjugated goat anti-rabbit IgG (1:1000, A0562, Beyotime, Shanghai, China) or Cy3-conjugated goat anti-mouse IgG (1:1000, A0521, Beyotime, Shanghai, China) or goat anti-mouse IgG conjugated with 594 (33212ES60, Yeasen, Shanghai, China). .. Cell nuclei of the tissue were stained with fluorescence quenching anti-fade mounting solution containing DAPI (P0126, Beyotime, Shanghai, China). ..

    Article Title: Host protein eEF1A1 affected the infection of Cryptosporidium parvum through interacting with CpHSP90
    Article Snippet: Plasmids pBiFC-VN155-CpHSP90 and pBiFC-VC155-eEF1A1 were transfected into HEK293T cells on confocal culture dishes (Biosharp, Hefei, China), with established negative and positive controls. .. At 24 h post-transfection, the medium was removed, and the cells were washed three times with PBS, and fixed with 200 μL of 4% paraformaldehyde (Biosharp) at room temperature for 10 min. After three washes with PBS, cells were stained using an anti-fade mounting solution containing DAPI (Beyotime). .. Images were captured using an Olympus IX83 P2ZF super-resolution microscope (Olympus, Tokyo, Japan).

    Incubation:

    Article Title: Targeted activation of ErbB4 receptor ameliorates neuronal deficits and neuroinflammation in a food-borne polystyrene microplastic exposed mouse model.
    Article Snippet: Fixed microglial cells were treated with mouse anti-IBA1 antibody (1:100, GB15105-100, Servicebio, Wuhan, China) in combination with rabbit anti-phosphorylated nuclear factor-Kappa B (p-NF-κB) antibody (1:200, GB113882-100, Servicebio, Wuhan, China). .. After being rinsed 3 times with PBS (5 min each), the cells were incubated with a mixture of Alexafluor 488-conjugated goat anti-mouse IgG (1:200, BA1126, Boster, Wuhan, China) and Alexafluor 594-conjugated goat anti-rabbit IgG (1:200, BA1142, Boster, Wuhan, China) for 2 h. Samples were subsequently mounted using an anti-fade mounting solution containing DAPI (P0131, Beyotime, Shanghai, China). .. Images were finally acquired using a fluorescence microscope (Axio Imager Z2, Carl Zeiss AG, DE).

    Article Title: Targeted activation of ErbB4 receptor ameliorates neuronal deficits and neuroinflammation in a food-borne polystyrene microplastic exposed mouse model
    Article Snippet: Fixed microglial cells were treated with mouse anti-IBA1 antibody (1:100, GB15105-100, Servicebio, Wuhan, China) in combination with rabbit anti-phosphorylated nuclear factor-Kappa B (p-NF-κB) antibody (1:200, GB113882-100, Servicebio, Wuhan, China). .. After being rinsed 3 times with PBS (5 min each), the cells were incubated with a mixture of Alexafluor 488-conjugated goat anti-mouse IgG (1:200, BA1126, Boster, Wuhan, China) and Alexafluor 594-conjugated goat anti-rabbit IgG (1:200, BA1142, Boster, Wuhan, China) for 2 h. Samples were subsequently mounted using an anti-fade mounting solution containing DAPI (P0131, Beyotime, Shanghai, China). .. Images were finally acquired using a fluorescence microscope (Axio Imager Z2, Carl Zeiss AG, DE).

    Fluorescence:

    Article Title: Akkermansia muciniphila Is Beneficial to a Mouse Model of Parkinson’s Disease, via Alleviated Neuroinflammation and Promoted Neurogenesis, with Involvement of SCFAs
    Article Snippet: The sections were washed three times in PBS and treated for 1 h at 37 °C with a secondary antibody coupled to FITC-conjugated goat anti-rabbit IgG (1:1000, A0562, Beyotime, Shanghai, China) or Cy3-conjugated goat anti-mouse IgG (1:1000, A0521, Beyotime, Shanghai, China) or goat anti-mouse IgG conjugated with 594 (33212ES60, Yeasen, Shanghai, China). .. Cell nuclei of the tissue were stained with fluorescence quenching anti-fade mounting solution containing DAPI (P0126, Beyotime, Shanghai, China). ..



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